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Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension

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The Surfaceome

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1722))

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Abstract

Enrichment of the plasma membrane represents one valuable method to characterize the surfaceome, along with other plasma membrane and structural proteins. Currently, the overlapping densities of many subcellular organelles hinder enrichment of the plasma membrane by centrifugation. However, external access to the plasma membrane of intact cells allows the attachment of a nanoparticle pellicle to enhance its density and facilitate enrichment. We describe the synthesis of iron oxide nanoparticles, attachment of the pellicle to suspended cells, and recovery of plasma membrane proteins for proteomic analysis.

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Acknowledgment

This developmental research was supported by a grant from the US National Institutes of Health, GM021248.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA

    Rebecca L. Rose & Catherine Fenselau

  2. Department of Biochemistry, Faculty of Science, Burapha University, Mueang, Chonburi, Thailand

    Waeowalee Choksawangkarn

Authors
  1. Rebecca L. Rose
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  2. Waeowalee Choksawangkarn
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  3. Catherine Fenselau
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Corresponding author

Correspondence to Catherine Fenselau .

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Editors and Affiliations

  1. School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

    Kenneth R. Boheler

  2. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA

    Rebekah L. Gundry

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Rose, R.L., Choksawangkarn, W., Fenselau, C. (2018). Application of Higher Density Iron Oxide Nanoparticle Pellicles to Enrich the Plasma Membrane and Its Proteome from Cells in Suspension. In: Boheler, K., Gundry, R. (eds) The Surfaceome. Methods in Molecular Biology, vol 1722. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7553-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7553-2_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7551-8

  • Online ISBN: 978-1-4939-7553-2

  • eBook Packages: Springer Protocols

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