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The Surfaceome pp 185–194Cite as

High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1722))

Abstract

Considerable interest has been raised to develop human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) as a model for drug discovery and cardiotoxicity screening. High-content electrophysiological analysis of currents generated by transmembrane cell surface ion channels has been pursued to complement such emerging applications. Here we describe practical procedures and considerations for accomplishing successful assays of hPSC-CMs using an automated planar patch-clamp system.

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Acknowledgements

This work was supported by the Theme-based Research Scheme [T13-706/11] of Hong Kong Research Grant Council.

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Authors and Affiliations

  1. Stem Cell & Regenerative Medicine Consortium, Department of Paediatrics and Adolescent Medicine, School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

    Chi-Wing Kong

  2. Dr. Li Dak-Sum Research Centre, University of Hong Kong, Hong Kong, SAR, China

    Ronald A. Li

  3. Ming-Wai Lau Centre for Reparative Medicine, Karolinska Institutet, Stockholm, Sweden

    Ronald A. Li

  4. Department of Paediatrics and Adolescent Medicine, School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

    Lin Geng

Authors
  1. Chi-Wing Kong
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  2. Lin Geng
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  3. Ronald A. Li
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Corresponding author

Correspondence to Chi-Wing Kong .

Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

    Kenneth R. Boheler

  2. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA

    Rebekah L. Gundry

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Kong, CW., Geng, L., Li, R.A. (2018). High-Content Electrophysiological Analysis of Human Pluripotent Stem Cell-Derived Cardiomyocytes (hPSC-CMs). In: Boheler, K., Gundry, R. (eds) The Surfaceome. Methods in Molecular Biology, vol 1722. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7553-2_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7553-2_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7551-8

  • Online ISBN: 978-1-4939-7553-2

  • eBook Packages: Springer Protocols

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