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The Surfaceome pp 3–20Cite as

Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1722))

Abstract

The bacterial surfaceome, comprising outer membrane-sorted and/or associated (i.e., cell transporters), cell surface-exposed (i.e., adhesins) and extracellularly secreted proteins (i.e., toxins), has been characterized in bacterial pathogens, such as Bordetella pertussis (Bp) to provide information for use in development of diagnostic and prevention strategies. This protein subset has clinical significance, as these bacterial proteins are often associated with attachment to host cells, microbial pathogenesis and antibody-mediated immunity. Here we describe classical surface membrane protein enrichment techniques, followed by proteomic methodologies, such as gel-free protein separation and antibody-affinity capture technologies in combination with nano-liquid chromatography mass spectrometry, for the identification and characterization of Bp surfaceome proteins.

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Acknowledgments

The authors would like to thank Drs. Jacquelyn Sampson, Eddie Ades, Maria L. Tondella, and George Carlone at the Centers for Disease Control and Prevention for their insight and providing materials associated with the described research protocols.

Disclaimer

References in this chapter to any specific commercial products, process, service, manufacturer, or company do not constitute an endorsement or a recommendation by the US Government or the Centers for Disease Control and Prevention. The described protocol and suggested notes in this report are those of the authors and do not necessarily represent the views of CDC.

Author information

Authors and Affiliations

  1. Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, Chamblee, GA, USA

    Yulanda M. Williamson, Hercules Moura, Adrian R. Woolfitt, Jon Rees, David M. Schieltz & John R. Barr

  2. Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA

    Jennifer Whitmon & Rolieria West-Deadwyler

Authors
  1. Yulanda M. Williamson
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  2. Jennifer Whitmon
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  3. Rolieria West-Deadwyler
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  4. Hercules Moura
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  5. Adrian R. Woolfitt
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  6. Jon Rees
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  7. David M. Schieltz
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  8. John R. Barr
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Corresponding author

Correspondence to John R. Barr .

Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, SAR, China

    Kenneth R. Boheler

  2. Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA

    Rebekah L. Gundry

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Cite this protocol

Williamson, Y.M. et al. (2018). Surfaceome Analysis Protocol for the Identification of Novel Bordetella pertussis Antigens. In: Boheler, K., Gundry, R. (eds) The Surfaceome. Methods in Molecular Biology, vol 1722. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7553-2_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7553-2_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7551-8

  • Online ISBN: 978-1-4939-7553-2

  • eBook Packages: Springer Protocols

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