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Extracellular Recording Approaches pp 39–71Cite as

Single-Unit Extracellular Recording from the Cerebellum During Eyeblink Conditioning in Head-Fixed Mice

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Part of the book series: Neuromethods ((NM,volume 134))

Abstract

This chapter presents a method for performing in vivo single-unit extracellular recordings and optogenetics during an associative, cerebellum-dependent learning task in head-fixed mice. The method uses a cylindrical treadmill system that reduces stress in the mice by allowing them to walk freely, yet it provides enough stability to maintain single-unit isolation of neurons for tens of minutes to hours. Using this system, we have investigated sensorimotor coding in the cerebellum while mice perform learned skilled movements.

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Authors and Affiliations

  1. Department of Neuroscience, Baylor College of Medicine, Houston, TX, USA

    Shane A. Heiney, Shogo Ohmae, Olivia A. Kim & Javier F. Medina

Authors
  1. Shane A. Heiney
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  2. Shogo Ohmae
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  3. Olivia A. Kim
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  4. Javier F. Medina
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Corresponding author

Correspondence to Javier F. Medina .

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Editors and Affiliations

  1. Department of Pathology & Immunology and Neuroscience, Baylor College of Medicine, Houston, Texas, USA

    Roy V. Sillitoe

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Heiney, S.A., Ohmae, S., Kim, O.A., Medina, J.F. (2018). Single-Unit Extracellular Recording from the Cerebellum During Eyeblink Conditioning in Head-Fixed Mice. In: Sillitoe, R. (eds) Extracellular Recording Approaches. Neuromethods, vol 134. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7549-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7549-5_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7548-8

  • Online ISBN: 978-1-4939-7549-5

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