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iCLIP of the PIWI Protein Aubergine in Drosophila Embryos

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Book cover mRNA Decay

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1720))

Abstract

Piwi-interacting RNAs (piRNAs) are a class of small noncoding RNAs bound to specific Argonaute proteins, the PIWI proteins. piRNAs target mRNAs by complementarity to silence them; they play an important role in the repression of transposable elements in the germ line of many species. piRNAs and PIWI proteins are also involved in diverse biological processes through their role in the regulation of cellular mRNAs. In the Drosophila embryo, they contribute to the maternal mRNA decay occurring during the maternal-to-zygotic transition. CLIP (UV cross-linking and immunoprecipitation) techniques have been used to identify target mRNAs of Argonaute proteins. Here we describe the iCLIP (individual-nucleotide resolution CLIP) protocol that we have adapted for the PIWI protein Aubergine in Drosophila embryos.

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Acknowledgments

Work in the Simonelig lab is supported by the UMR9002 CNRS-Université de Montpellier, ANR (ANR-15- CE12-0019-01), FRM (Equipe FRM 2013 DEQ20130326534), and AFM-Telethon (N° 17110). B.B. was supported by D.F.G. and J.D. by Fondation ARC.

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Authors and Affiliations

  1. mRNA Regulation and Development, Institute of Human Genetics, UMR9002 CNRS-Université de Montpellier, Montpellier Cedex 5, France

    Bridlin Barckmann, Jérémy Dufourt & Martine Simonelig

Authors
  1. Bridlin Barckmann
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  2. Jérémy Dufourt
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  3. Martine Simonelig
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Correspondence to Martine Simonelig .

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Editors and Affiliations

  1. Murdoch Children’s Research Institute, The Royal Children’s Hospital, Parkville, Victoria, Australia

    Shireen R. Lamandé

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Barckmann, B., Dufourt, J., Simonelig, M. (2018). iCLIP of the PIWI Protein Aubergine in Drosophila Embryos. In: Lamandé, S. (eds) mRNA Decay. Methods in Molecular Biology, vol 1720. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7540-2_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7540-2_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7539-6

  • Online ISBN: 978-1-4939-7540-2

  • eBook Packages: Springer Protocols

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