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mRNA Decay pp 141–148Cite as

Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1720))

Abstract

miRNAs are involved in various biological processes and different diseases through interacting with their target mRNAs. Therefore, deciphering miRNA targets is important for diagnostics and therapeutics. However, it is still complex and expensive to do large scale screening for miRNA alterations and identify their downstream miRNA targets. Recently, integrating analysis of miRNA expression profiles and mRNA expression levels has been used to successfully identify the most prominent interactions. Here, we present a protocol that combines both expression data and computational prediction to refine the microarray result for identifying the miRNA targets. Many details are also provided to help researchers choose more appropriate tools and methods for a given type of application. Available web-based resources and experimental conditions for functional enrichment and validation of miRNA targets are also summarized.

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Acknowledgment

This work was supported by the Natural Science Foundation of Fujian Province (No. 2016J01171) of China.

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Authors and Affiliations

  1. Translational Medicine Research Center, School of Pharmaceutical Sciences, Xiamen University, Xiamen, Fujian, China

    Zhiming Li & Chi-Meng Tzeng

  2. Key Laboratory for Cancer T-Cell Theranostics and Clinical Translation (CTCTCT), Xiamen, Fujian, China

    Zhiming Li & Chi-Meng Tzeng

  3. INNOVA Clinics and TRANSLA Health Group University, Xiamen, Fujian, China

    Zhiming Li & Chi-Meng Tzeng

Authors
  1. Zhiming Li
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  2. Chi-Meng Tzeng
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Corresponding author

Correspondence to Chi-Meng Tzeng .

Editor information

Editors and Affiliations

  1. Murdoch Children’s Research Institute, The Royal Children’s Hospital, Parkville, Victoria, Australia

    Shireen R. Lamandé

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Li, Z., Tzeng, CM. (2018). Integrated Analysis of miRNA and mRNA Expression Profiles to Identify miRNA Targets. In: Lamandé, S. (eds) mRNA Decay. Methods in Molecular Biology, vol 1720. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7540-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7540-2_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7539-6

  • Online ISBN: 978-1-4939-7540-2

  • eBook Packages: Springer Protocols

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