Skip to main content
SpringerLink
Log in
Book cover

Peptidomics pp 349–358Cite as

Analysis of the Snake Venom Peptidome

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1719))

Abstract

Snake venom peptidomes are known to be a large source of molecules with different pharmacological properties. The complexity and variability of snake venoms, the presence of proteinases, and the lack of complete species-specific genome sequences make snake venom peptidome profiling a challenging task that requires especial technical strategies for sample processing and mass spectrometric analysis. Here we describe a method for assessing the content of snake venom peptides and highlight the importance of sampling procedures, as they substantially influence the peptidomic complexity of snake venoms.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   159.00
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Murayama N, Hayashi MA, Ohi H, Ferreira LA, Hermann VV, Saito H, Fujita Y, Higuchi S, Fernandes BL, Yamane T, de Camargo AC (1997) Cloning and sequence analysis of a Bothrops jararaca cDNA encoding a precursor of seven bradykinin-potentiating peptides and a C-type natriuretic peptide. Proc Natl Acad Sci U S A 94:1189–1193

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Camargo AC, Ianzer D, Guerreiro JR, Serrano SM (2012) Bradykinin-potentiating peptides: beyond captopril. Toxicon 59:516–523

    Article  CAS  PubMed  Google Scholar 

  3. Fry BG, Vidal N, Norman JA, Vonk FJ, Scheib H, Ramjan SF, Kuruppu S, Fung K, Hedges SB, Richardson MK, Hodgson WC, Ignjatovic V, Summerhayes R, Kochva E (2006) Early evolution of the venom system in lizards and snakes. Nature 439:584–588

    Article  CAS  PubMed  Google Scholar 

  4. Wagstaff SC, Favreau P, Cheneval O, Laing GD, Wilkinson MC, Miller RL, Stöcklin R, Harrison RA (2008) Molecular characterisation of endogenous snake venom metalloproteinase inhibitors. Biochem Biophys Res Commun 365:650–656

    Article  CAS  PubMed  Google Scholar 

  5. Munawar A, Trusch M, Georgieva D, Spencer P, Frochaux V, Harder S, Arni RK, Duhalov D, Genov N, Schlüter H, Betzel C (2011) Venom peptide analysis of Vipera ammodytes meridionalis (Viperinae) and Bothrops jararacussu (Crotalinae) demonstrates subfamily-specificity of the peptidome in the family Viperidae. Mol BioSyst 7:3298–3307

    Article  CAS  PubMed  Google Scholar 

  6. Tashima AK, Zelanis A, Kitano ES, Ianzer D, Melo RL, Rioli V, Sant’anna SS, Schenberg AC, Camargo AC, Serrano SM (2012) Peptidomics of three Bothrops snake venoms: insights into the molecular diversification of proteomes and peptidomes. Mol Cell Proteomics 11:1245–1262

    Article  PubMed  PubMed Central  Google Scholar 

  7. Zelanis A, Tashima AK (2014) Unraveling snake venom complexity with ‘omics’ approaches: challenges and perspectives. Toxicon 87:131–134

    Article  CAS  PubMed  Google Scholar 

  8. Nicolau CA, Carvalho PC, Junqueira-de-Azevedo IL, Teixeira-Ferreira A, Junqueira M, Perales J, Neves-Ferreira AG, Valente RH (2016) An in-depth snake venom proteopeptidome characterization: benchmarking Bothrops jararaca. J Proteomics 151:211–231

    Google Scholar 

  9. Pimenta DC, Prezoto BC, Konno K, Melo RL, Furtado MF, Camargo AC, Serrano SM (2007) Mass spectrometric analysis of the individual variability of Bothrops jararaca venom peptide fraction. Evidence for sex-based variation among the bradykinin-potentiating peptides. Rapid Commun Mass Spectrom 21:1034–1042

    Article  CAS  PubMed  Google Scholar 

  10. Zelanis A, Tashima AK, Rocha MM, Furtado MF, Camargo AC, Ho PL, Serrano SM (2010) Analysis of the ontogenetic variation in the venom proteome/peptidome of Bothrops jararaca reveals different strategies to deal with prey. J Proteome Res 9:2278–2291

    Article  CAS  PubMed  Google Scholar 

  11. Zelanis A, Menezes MC, Kitano ES, Liberato T, Tashima AK, Pinto AF, Sherman NE, Ho PL, Fox JW, Serrano SM (2016) Proteomic identification of gender molecular markers in Bothrops jararaca venom. J Proteome 139:26–37

    Article  CAS  Google Scholar 

  12. Tashima AK, Zelanis A (2016) Snake venom peptidomics. In: Gopalakrishnakone P, Calvete JJ (eds) Venom genomics and proteomics. Springer, Dordrecht, pp 317–331

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-Response and Cell Signaling (CeTICS), Instituto Butantan, São Paulo, SP, Brazil

    Solange M. T. Serrano & Eduardo S. Kitano

  2. Instituto de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos Campos, SP, Brazil

    André Zelanis

  3. Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil

    Alexandre K. Tashima

Authors
  1. Solange M. T. Serrano
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. André Zelanis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eduardo S. Kitano
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Alexandre K. Tashima
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Solange M. T. Serrano .

Editor information

Editors and Affiliations

  1. Department of Biotechnology and Bioinformatics, Weihenstephan-Tr. University of Applied Sciences, Freising, Bayern, Germany

    Michael Schrader

  2. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York, USA

    Lloyd Fricker

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Serrano, S.M.T., Zelanis, A., Kitano, E.S., Tashima, A.K. (2018). Analysis of the Snake Venom Peptidome. In: Schrader, M., Fricker, L. (eds) Peptidomics. Methods in Molecular Biology, vol 1719. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7537-2_23

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7537-2_23

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7536-5

  • Online ISBN: 978-1-4939-7537-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation