Diffusion-Weighted Magnetic Resonance Imaging

  • Irene Guadilla
  • Daniel Calle
  • Pilar López-LarrubiaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1718)


Magnetic resonance imaging (MRI) is a technique based on the contents and relaxation features of water in tissues. In basic MRI sequences, diffusion phenomenon of water molecules is not taken into account although it has a notable influence in the relaxation times, and therefore in the signal intensity of images. In fact, MRI techniques that take advantage of water diffusion have experienced a huge development in last years. Diffusion-weighted imaging (DWI) has spectacularly evolved reaching nowadays a great impact both in clinical and preclinical imaging—especially in the neuroimaging field—and in basic research. We present here a protocol to perform DWI studies in a high-field preclinical setup.

Key words

Magnetic resonance imaging Preclinical MRI Diffusion-weighted imaging Echo-planar imaging Apparent diffusion coefficient 



This work was supported by grant SAF2014-53739-R. IG held a predoctoral contract from Ministerio de Economía, Indrustria y Competitividad (MINECO) of Spain. DC held a postdoctoral contract from Consejo Superior de Investigaciones Científicas (CSIC).


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Irene Guadilla
    • 1
  • Daniel Calle
    • 1
  • Pilar López-Larrubia
    • 1
    Email author
  1. 1.Instituto de Investigaciones Biomédicas “Alberto Sols”CSIC/UAMMadridSpain

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