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Preclinical MRI pp 285–296Cite as

In Utero MRI of Mouse Embryos

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1718))

Abstract

Genetically engineered mouse models are used extensively as models of human development and developmental diseases. Conventional histological approaches are static and two-dimensional, and do not provide a full understanding of the dynamic, spatiotemporal changes in developing mouse embryos. Magnetic resonance imaging (MRI) offers a noninvasive and longitudinal approach for three-dimensional in utero imaging of normal and mutant mouse embryos. In this chapter, we describe MRI approaches that have been developed for imaging the living embryonic mouse brain and vasculature. Details are provided on the animal preparation and setup, MRI equipment, acquisition and reconstruction methods that have been found to be most useful for in utero MRI, including examples of applications to fetal mouse neuroimaging.

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Acknowledgements

We thank all the people, current and past, in the Zhang, Wu and Turnbull labs who have contributed to developing the protocols described in this chapter. This research was supported, in part, by grants from the National Institutes of Health: R01NS038461 and R01HL078665 (DHT); R01NS070909 and R01HD974593 (JZ); R21NS098018 (DW).

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Authors and Affiliations

  1. Department of Radiology, Bernard and Irene Schwartz Center for Biomedical Imaging, New York University (NYU) School of Medicine, New York, NY, USA

    Jiangyang Zhang & Daniel H. Turnbull

  2. Department of Radiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA

    Dan Wu

  3. Department of Pathology, NYU School of Medicine, New York, NY, USA

    Daniel H. Turnbull

  4. Kimmel Center for Biology and Medicine at the Skirball Institute of Biomolecular Medicine, NYU School of Medicine, New York, NY, USA

    Daniel H. Turnbull

Authors
  1. Jiangyang Zhang
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  2. Dan Wu
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  3. Daniel H. Turnbull
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Corresponding author

Correspondence to Daniel H. Turnbull .

Editor information

Editors and Affiliations

  1. BIONAND, Andalusian Centre for Nanomedicine and Biotechnology, Junta de Andalucía, Universidad de Málaga, Málaga, Spain

    María Luisa García Martín

  2. Instituto de Investigaciones Biomédicas “Alberto Sols”, CSIC/UAM, Madrid, Spain

    Pilar López Larrubia

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Zhang, J., Wu, D., Turnbull, D.H. (2018). In Utero MRI of Mouse Embryos. In: García Martín, M., López Larrubia, P. (eds) Preclinical MRI. Methods in Molecular Biology, vol 1718. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7531-0_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7531-0_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7530-3

  • Online ISBN: 978-1-4939-7531-0

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