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Biomedical 19F MRI Using Perfluorocarbons

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Preclinical MRI

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1718))

Abstract

Background-free fluorine (19F) MR imaging exhibits an excellent degree of specificity, and facilitates among others the in vivo visualization of inflammatory processes. Merging19F MR images with morphologically matching1H MR images enables the exact anatomic localization of the observed19F signal. Biochemically inert nanoemulsions of perfluorocarbons, which are known to be taken up by the macrophage/monocyte system, are widely used as contrast agents for preclinical applications. Herein, the most common protocols are described to obtain high-resolution and artifact-free19F MR images even for compounds with complex19F MR spectra. In addition, we report on the utilization of perfluorocarbons with individual spectral identities and targeting approaches to specifically visualize thrombi by19F MRI.

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Acknowledgements

The authors would like to thank Prof. Jürgen Schrader (Düsseldorf) for his continuous support and encouragement as well as Prof. Rolf Schubert and Dr. Christoph Grapentin (Freiburg) for their enormous help in the development of the perfluorocarbon emulsions (PFCs). Furthermore, we would like to thank Prof. Cornelia Blume (Hannover) for providing us with the glomerulonephritis model. The work shown herein was supported financially by the Deutsche Forschungsgemeinschaft (DFG), the subproject Z2 of the SFB 612, subprojects B2 and B5 of the SFB 1116, and grants SCHR 154/13-1+2.

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Author notes

  1. Tuba Güden-Silber and Sebastian Temme contributed equally to this work.

Authors and Affiliations

  1. Experimental Cardiovascular Imaging, Department of Molecular Cardiology, Heinrich Heine University, Düsseldorf, Germany

    Tuba Güden-Silber, Sebastian Temme, Christoph Jacoby & Ulrich Flögel

Authors
  1. Tuba Güden-Silber
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  2. Sebastian Temme
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  3. Christoph Jacoby
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  4. Ulrich Flögel
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Corresponding author

Correspondence to Ulrich Flögel .

Editor information

Editors and Affiliations

  1. BIONAND, Andalusian Centre for Nanomedicine and Biotechnology, Junta de Andalucía, Universidad de Málaga, Málaga, Spain

    María Luisa García Martín

  2. Instituto de Investigaciones Biomédicas “Alberto Sols”, CSIC/UAM, Madrid, Spain

    Pilar López Larrubia

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Güden-Silber, T., Temme, S., Jacoby, C., Flögel, U. (2018). Biomedical 19F MRI Using Perfluorocarbons. In: García Martín, M., López Larrubia, P. (eds) Preclinical MRI. Methods in Molecular Biology, vol 1718. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7531-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7531-0_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7530-3

  • Online ISBN: 978-1-4939-7531-0

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