1H Spectroscopic Imaging of the Rodent Brain

  • Rui V. SimõesEmail author
  • Emma Muñoz-Moreno
  • Raúl Tudela
  • Guadalupe Soria
Part of the Methods in Molecular Biology book series (MIMB, volume 1718)


Proton MR spectroscopic imaging (MRSI) can provide a variety of “molecular images” from animal models of human disease, which are useful for different research purposes. This chapter describes a protocol for in vivo acquisition and analysis of MRSI data from the rodent brain.

Key words

Magnetic Resonance Spectroscopic Imaging (MRSI) Chemical shift imaging (CSI) Field of view (FOV) Volume of interest (VOI) Point resolved spectroscopy (PRESS) Acquisition-weighting Spectral analysis 



This work was sponsored by grants from: La Caixa Foundation; AGAUR 2014 SGR, grant n° 928; RD12/0036/0010, integrated in Plan Nacional de I+D+I and co-funded by ISCIII-Subdirección General de Evaluación and Fondo Europeo de Desarrollo Regional (FEDER) (PI14/00595); Fundació La Marató de TV3 (201441 10); and CERCA Programme/Generalitat de Catalunya. We are indebted to the Experimental MRI 7T Unit of the IDIBAPS, for the technical help.


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • Rui V. Simões
    • 1
    • 4
    Email author
  • Emma Muñoz-Moreno
    • 2
  • Raúl Tudela
    • 3
  • Guadalupe Soria
    • 2
    • 3
  1. 1.Fetal i+D Fetal Medicine Research Center, BCNatal-Barcelona Center for Maternal-Fetal and Neonatal Medicine (Hospital Clínic and Hospital Sant Joan de Déu), Institut Clínic de Ginecologia, Obstetricia i Neonatologia, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, and Centre for Biomedical Research on Rare Diseases (CIBER-ER)BarcelonaSpain
  2. 2.Experimental 7T MRI Unit, IDIBAPSBarcelonaSpain
  3. 3.Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN)BarcelonaSpain
  4. 4.Champalimaud FoundationLisbonPortugal

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