In Vivo 1H Magnetic Resonance Spectroscopy

  • M. Carmen Muñoz-Hernández
  • María Luisa García-MartínEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1718)


In vivo Magnetic Resonance Spectroscopy (MRS) allows the non-invasive detection and quantification of a number of metabolites from localized volumes within a living organism. MRS localization techniques can be divided into two main groups, single voxel and multi-voxel. Single voxel techniques provide the metabolic profile from a specific small volume, whereas multi-voxel techniques are used to obtain the spatial distribution of metabolites throughout a large volume subdivided into small contiguous voxels. This chapter describes standard protocols for the acquisition and processing of in vivo single voxel1H MRS data from the rodent brain.

Key words

Localized spectroscopy Single voxel 1H MRS Brain metabolites 



The MRI system used in this work has been funded by the Spanish Ministry of Science and Innovation (National Plan for Scientific Research, Development and Technological Innovation 2008-2011) and the European Regional Development Fund (PCT-420000-2010-3).


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  • M. Carmen Muñoz-Hernández
    • 1
  • María Luisa García-Martín
    • 1
    • 2
    Email author
  1. 1.BIONAND, Andalusian Centre for Nanomedicine and BiotechnologyJunta de Andalucía, Universidad de MálagaMálagaSpain
  2. 2.Networking Research Center on Bioengineering, Biomaterials and NanomedicineCIBER-BBNMálagaSpain

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