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Experimental Protocol for Cecal Ligation and Puncture Model of Polymicrobial Sepsis and Assessment of Vascular Functions in Mice

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Traumatic and Ischemic Injury

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1717))

Abstract

Sepsis is the systemic inflammatory response syndrome that occurs during infection and is exacerbated by the inappropriate immune response encountered by the affected individual. Despite extensive research, sepsis in humans is one of the biggest challenges for clinicians. The high mortality rate in sepsis is primarily due to hypoperfusion-induced multiorgan dysfunctions , resulting from a marked decrease in peripheral resistance. Vascular dysfunctions are further aggravated by sepsis-induced impairment in myocardial contractility. Circulatory failure in sepsis is characterized by refractory hypotension and vascular hyporeactivity (vasoplegia) to clinically used vasoconstrictors. To investigate the complex pathophysiology of sepsis and its associated multiple organ dysfunction, several animal models have been developed. However, cecal ligation and puncture (CLP) model of murine sepsis is still considered as ‘gold standard’ in sepsis research. In this protocol we have described the standard surgical procedure to induce polymicrobial sepsis by cecal ligation and puncture. Further, we have described the protocol to study the molecular mechanisms underlying vascular dysfunctions in sepsis.

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Author information

Authors and Affiliations

  1. Division of Pharmacology & Toxicology, Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India

    Santosh Kumar Mishra

  2. Bhubaneswar, Odisha, India

    Santosh Kumar Mishra

  3. Department of Pharmacology and Toxicology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, Uttar Pradesh, India

    Soumen Choudhury

Authors
  1. Santosh Kumar Mishra
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  2. Soumen Choudhury
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Corresponding author

Correspondence to Santosh Kumar Mishra .

Editor information

Editors and Affiliations

  1. Department of Surgery, Texas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research Institute, Temple, Texas, USA

    Binu Tharakan

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Mishra, S.K., Choudhury, S. (2018). Experimental Protocol for Cecal Ligation and Puncture Model of Polymicrobial Sepsis and Assessment of Vascular Functions in Mice. In: Tharakan, B. (eds) Traumatic and Ischemic Injury. Methods in Molecular Biology, vol 1717. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7526-6_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7526-6_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7524-2

  • Online ISBN: 978-1-4939-7526-6

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