Abstract
Generation of lentivirus (LV)-based vectors holding multiple gene cassettes for coexpression of several therapeutic factors provides potent tools in both gene delivery studies as well as in gene therapy. Here we describe the development of such multigenic LV gene delivery vectors enabling cell-specific coexpression of antiangiogenic microRNA (miRNA) and protein factors and, if preferred, a fluorescent reporter, from RNApol(II)-driven expression cassettes orientated in a back-to-back fashion. This configuration may contribute to the development of new combination therapies for amelioration of diseases involving intraocular neovascularization including exudative age-related macular degeneration (AMD).
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Acknowledgment
This work was supported by the Lundbeck Foundation (Grant No. R165-2013-15631), Gene Therapy Initiative Aarhus (GTI-Aarhus) funded by the Lundbeck Foundation (Grant No. R126-2012-12456), The Danish Eye Foundation, and Riisfort Fonden. The pLV/PE and CMV-β-globin intron bovine poly A vectors were generously provided by Professor Jacob Giehm Mikkelsen and Associate Professor Lars Aagaard (both Department of Biomedicine, Aarhus University, Denmark), respectively.
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Askou, A.L., Corydon, T.J. (2018). Development of Multigenic Lentiviral Vectors for Cell-Specific Expression of Antiangiogenic miRNAs and Protein Factors. In: Boon, C., Wijnholds, J. (eds) Retinal Gene Therapy. Methods in Molecular Biology, vol 1715. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7522-8_4
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DOI: https://doi.org/10.1007/978-1-4939-7522-8_4
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