During infection, the cytosolic detection of viral double-stranded RNA (dsRNA) leads to the oligomerization and activation of mitochondrial antiviral signaling protein (MAVS) and the subsequent production of type I interferon (IFN). Here, we describe a novel method of visualizing and quantifying the aggregation of MAVS in response to dsRNA stimulation or viral infection in vitro using confocal microscopy.
Mitochondrial antiviral signaling protein (MAVS) Confocal microscopy Type I interferon Double-stranded RNA Virus
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Loo Y-M, Fornek J, Crochet N et al (2008) Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity. J Virol 82:335–345CrossRefPubMedGoogle Scholar
Seth RB, Sun L, Ea C-K et al (2005) Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3. Cell 122:669–682CrossRefPubMedGoogle Scholar
Xu L-G, Wang Y-Y, Han K-J et al (2005) VISA is an adapter protein required for virus-triggered IFN-β signaling. Mol Cell 19:727–740CrossRefPubMedGoogle Scholar
Meylan E, Curran J, Hofmann K et al (2005) Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus. Nature 437:1167–1172CrossRefPubMedGoogle Scholar
Kawai T, Takahashi K, Sato S et al (2005) IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction. Nat Immunol 6:981–988CrossRefPubMedGoogle Scholar
Zhao Y, Sun X, Nie X et al (2012) COX5B regulates MAVS-mediated antiviral signaling through interaction with ATG5 and repressing ROS production. PLoS Pathog 8:e1003086CrossRefPubMedPubMedCentralGoogle Scholar