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Detection and Quantification of MAVS Aggregation via Confocal Microscopy

  • Tan A. Nguyen
  • Lachlan Whitehead
  • Ken C. PangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1714)

Abstract

During infection, the cytosolic detection of viral double-stranded RNA (dsRNA) leads to the oligomerization and activation of mitochondrial antiviral signaling protein (MAVS) and the subsequent production of type I interferon (IFN). Here, we describe a novel method of visualizing and quantifying the aggregation of MAVS in response to dsRNA stimulation or viral infection in vitro using confocal microscopy.

Keywords

Mitochondrial antiviral signaling protein (MAVS) Confocal microscopy Type I interferon Double-stranded RNA Virus 

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Tan A. Nguyen
    • 1
    • 2
  • Lachlan Whitehead
    • 1
    • 2
  • Ken C. Pang
    • 1
    • 3
    • 4
    • 5
    Email author
  1. 1.Inflammation DivisionThe Walter and Eliza Hall Institute of Medical ResearchParkvilleAustralia
  2. 2.Department of Medical BiologyUniversity of MelbourneParkvilleAustralia
  3. 3.Murdoch Childrens Research InstituteParkvilleAustralia
  4. 4.Department of PaediatricsUniversity of MelbourneParkvilleAustralia
  5. 5.Department of PsychiatryUniversity of MelbourneParkvilleAustralia

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