Cells present ancestral conserved mechanisms to maintain their volume in response to alterations in environmental osmolarity. Changes in environmental osmolarities are therefore conserved as primitive stress signals. Innate immune cells, such as macrophages, express receptors to respond and shape immune response to stress, damage, or infection. The NLRP3 inflammasome is a multiprotein complex expressed in macrophages that senses pathogen- and danger-associated signals. The basic mechanisms of cell swelling and regulatory volume decrease are sensed by the NLRP3 inflammasome. Here, we present methods to study NLRP3 inflammasome activation in response to cell swelling.
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This work was supported by the European Research Council (ERC-2013-CoG 614578), EU COST Program (BM1406) and instituto salud carlos III-FEDER grants to PP. VC was supported by the Institut National de la Santé et de la Recherche Médicale.
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