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Innate Immune Activation pp 191–197Cite as

Methods to Study Cell Swelling-Induced Inflammasome Activation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1714))

Abstract

Cells present ancestral conserved mechanisms to maintain their volume in response to alterations in environmental osmolarity. Changes in environmental osmolarities are therefore conserved as primitive stress signals. Innate immune cells, such as macrophages, express receptors to respond and shape immune response to stress, damage, or infection. The NLRP3 inflammasome is a multiprotein complex expressed in macrophages that senses pathogen- and danger-associated signals. The basic mechanisms of cell swelling and regulatory volume decrease are sensed by the NLRP3 inflammasome. Here, we present methods to study NLRP3 inflammasome activation in response to cell swelling.

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References

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Acknowledgments

This work was supported by the European Research Council (ERC-2013-CoG 614578), EU COST Program (BM1406) and instituto salud carlos III-FEDER grants to PP. VC was supported by the Institut National de la Santé et de la Recherche Médicale.

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Authors and Affiliations

  1. Institut de Génomique Fonctionnelle, Labex ICST, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5203, Université Montpellier, Montpellier, France

    Vincent Compan

  2. Institut National de la Santé et de la Recherche Médicale Unité 1191, Montpellier, France

    Vincent Compan

  3. Molecular Inflammation Unit, Biomedical Research Institute of Murcia (IMIB-Arrixaca), Clinical University Hospital Virgen de la Arrixaca, University of Murcia, LAIB building, Room 4.15, Carretera Buenavista, s/n, 30120 El Palmar, Murcia, Spain

    Pablo Pelegrín

Authors
  1. Vincent Compan
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  2. Pablo Pelegrín
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Corresponding author

Correspondence to Pablo Pelegrín .

Editor information

Editors and Affiliations

  1. Inflammation Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia

    Dominic De Nardo

  2. Chemical Biology Division, The Walter & Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia

    Christine M De Nardo

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Compan, V., Pelegrín, P. (2018). Methods to Study Cell Swelling-Induced Inflammasome Activation. In: De Nardo, D., De Nardo, C. (eds) Innate Immune Activation. Methods in Molecular Biology, vol 1714. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7519-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7519-8_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7518-1

  • Online ISBN: 978-1-4939-7519-8

  • eBook Packages: Springer Protocols

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