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Measuring Nascent Transcripts by Nascent-seq

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Next Generation Sequencing

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1712))

Abstract

A complete understanding of transcription and co-transcriptional RNA processing events by polymerase requires precise and robust approaches to visualize polymerase progress and quantify nascent transcripts on a genome-wide scale. Here, we present a transcriptome-wide method to measure the level of nascent transcribing RNA in a fast and unbiased manner.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Searle 6-510, 320 E. Superior St., Chicago, IL, 60611, USA

    Fei Xavier Chen, Stacy A. Marshall, Yu Deng & Sun Tianjiao

Authors
  1. Fei Xavier Chen
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  2. Stacy A. Marshall
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  3. Yu Deng
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  4. Sun Tianjiao
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Corresponding author

Correspondence to Fei Xavier Chen .

Editor information

Editors and Affiliations

  1. Next Generation Sequencing and Microarray Core Facility, The Scripps Research Institute, La Jolla, California, USA

    Steven R. Head

  2. Next Generation Sequencing and Microarray Core Facility, The Scripps Research Institute, La Jolla, California, USA

    Phillip Ordoukhanian

  3. Department of Molecular Medicine, The Scripps Research Institute Center for Organ and Cell Transplantation, La Jolla, California, USA

    Daniel R. Salomon

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Chen, F.X., Marshall, S.A., Deng, Y., Tianjiao, S. (2018). Measuring Nascent Transcripts by Nascent-seq. In: Head, S., Ordoukhanian, P., Salomon, D. (eds) Next Generation Sequencing. Methods in Molecular Biology, vol 1712. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7514-3_2

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  • DOI: https://doi.org/10.1007/978-1-4939-7514-3_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7512-9

  • Online ISBN: 978-1-4939-7514-3

  • eBook Packages: Springer Protocols

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