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Co-culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-placental Interactions: Focus on Estrogen Biosynthesis

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Book cover Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

Estrogens are produced in large amounts during pregnancy, as a result of a tightly regulated cooperation between the maternal and fetal adrenal cortex, which produce androgen precursors, and the placental villous trophoblast, which transforms these precursors into estrogens. These estrogens play an important role in proper placental function, in adaptation of the mother to pregnancy, as well as in adequate fetal development. Disruption of estrogen production is associated with poor pregnancy outcomes and fetal malformation or altered fetal programming. Pregnant women may be exposed to endocrine disruptors from environmental sources or medications, and it is crucial to study the effects of such compounds on feto-placental steroidogenesis. The H295R/BeWo co-culture model offers the opportunity to study these interactions, by making it possible to evaluate the effects of chemical exposures on androgen and estrogen biosynthesis, as well as on various other aspects of feto-placental communication.

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Acknowledgments

This work was financially supported by the Réseau de recherche en santé environnementale as part of the Fonds de recherche du Québec (FRQ)-Santé (C.V., J.T.S.), the Natural Sciences and Engineering Research Council of Canada (NSERC) grants 313312-2012 (J.T.S.) and 262011-2009 (C.V.), the March of Dimes Foundation (C.V.), as well as studentship awards to A.A.H.T. from NSERC, FRQ-Nature et Technologies, FRQ-Santé, and Canadian Institutes of Health Research.

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Author notes

  1. J. Thomas Sanderson and Cathy Vaillancourt share joint senior authorship and contributed equally to this work.

Authors and Affiliations

  1. INRS – Institut Armand-Frappier, 531, Boulevard des Prairies, Laval, QC, Canada, H7V 1B7

    Andrée-Anne Hudon Thibeault, J. Thomas Sanderson & Cathy Vaillancourt

  2. BioMed Research Centre, Laval, QC, Canada

    Cathy Vaillancourt

  3. BioMed Research Centre, Université du Québec à Montréal, C.P. 8888, succ. Centre-ville, Montréal, QC, Canada, H3C 3P8

    Andrée-Anne Hudon Thibeault

  4. Center for Interdisciplinary Research on Well-Being, Health, Society and Environment (CINBIOSE), Université du Québec à Montréal, C.P. 8888, succ. Centre-ville, Montréal, QC, Canada, H3C 3P8

    Andrée-Anne Hudon Thibeault & Cathy Vaillancourt

Authors
  1. Andrée-Anne Hudon Thibeault
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  2. J. Thomas Sanderson
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  3. Cathy Vaillancourt
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Corresponding authors

Correspondence to J. Thomas Sanderson or Cathy Vaillancourt .

Editor information

Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Thibeault, AA.H., Sanderson, J.T., Vaillancourt, C. (2018). Co-culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-placental Interactions: Focus on Estrogen Biosynthesis. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_23

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_23

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

  • eBook Packages: Springer Protocols

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