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Measurement of Oxidative Stress: Mitochondrial Function Using the Seahorse System

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Book cover Preeclampsia

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1710))

Abstract

The Seahorse XFp Analyzer is a powerful tool for the assessment of various parameters of cellular respiration. Here we describe the process of the Seahorse Cell Phenotype Test using the Seahorse XFp Analyzer to characterize the metabolic phenotype of live cells. The Seahorse XFp Analyzer can also be coupled with other assays to measure cellular energetics. Given that mitochondrial dysfunction is implicated in preeclampsia, the Seahorse XFp Analyzer will serve as a useful tool for the understanding of pathological metabolism in this disorder.

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References

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Acknowledgment

D.L. is supported by the Endocrine Society of Australia through a Research Higher Degree Scholarship. S.C. is supported by the Ovarian Cancer Research Foundation and the Granulosa Cell Tumor of the Ovary Foundation. The Hudson Institute is supported by the Victorian Government’s Operational Infrastructure Scheme.

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Authors and Affiliations

  1. Hudson Institution of Medical Research, Monash University, Clayton, VIC, 3168, Australia

    Dilys T. H. Leung & Simon Chu

  2. Department of Molecular and Translational Research, Monash University, Clayton, VIC, 3168, Australia

    Dilys T. H. Leung & Simon Chu

Authors
  1. Dilys T. H. Leung
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  2. Simon Chu
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Corresponding author

Correspondence to Simon Chu .

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Editors and Affiliations

  1. Monash Medical Centre, Monash University, Clayton, Victoria, Australia

    Padma Murthi

  2. INRS-Institut Armand-Frappier, Laval, Québec, Canada

    Cathy Vaillancourt

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Leung, D.T.H., Chu, S. (2018). Measurement of Oxidative Stress: Mitochondrial Function Using the Seahorse System. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7498-6_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7497-9

  • Online ISBN: 978-1-4939-7498-6

  • eBook Packages: Springer Protocols

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