Abstract
Exosomes are small (~100 nm) vesicles that carry a wide range of molecules including proteins, RNAs, and DNA. Exosomes are secreted from a wide range of cells including placental cells. Interestingly, exosomes secreted from placental cells have been identified in maternal circulation as early as in 6 weeks of gestation, and their concentration increases with the gestational age. While there is growing interest in elucidating the role of exosomes during normal and complicated pregnancies (such as preeclampsia), progress in the field has been delayed because of the inability to isolate placental exosomes from maternal circulation. Therefore, here we describe a workflow to isolate placental exosomes from maternal circulation.
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Acknowledgment
CS was in receipt of a Lions Medical Research Foundation Fellowship. This study was supported by The Lions Medical Research Foundation, UQ ECR award, Royal Brisbane and Women’s Foundation, Diabetes Australia, and UQ-Ochsner Seed Grant. The ISO17025 accredited research facility was supported by grants from Therapeutics Innovation Australia and the National Collaborative Research Infrastructure Strategy.
This review is supported partly by funding from the Lions Medical Research Foundation (LMRF), The University of Queensland, and Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT 1170809), Chile.
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Lai, A., Elfeky, O., Rice, G.E., Salomon, C. (2018). Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation. In: Murthi, P., Vaillancourt, C. (eds) Preeclampsia . Methods in Molecular Biology, vol 1710. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7498-6_10
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DOI: https://doi.org/10.1007/978-1-4939-7498-6_10
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