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DNA Methylation Protocols pp 123–136Cite as

Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1708))

Abstract

Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.

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References

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Acknowledgments

This work was supported by Research Program of Innovative Cell Biology by Innovative Technology (Cell Innovation) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and the Platform Project for Supporting in Drug Discovery and Life Science Research (Platform for Drug Discovery, Informatics and Structural Life Science) of AMED.

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Authors and Affiliations

  1. Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Fumihito Miura & Takashi Ito

  2. Core Research for Evolutional Science and Technology (CREST), Japan Agency for Medical Research and Development (AMED), 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Fumihito Miura & Takashi Ito

  3. Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Fumihito Miura

Authors
  1. Fumihito Miura
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  2. Takashi Ito
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Corresponding author

Correspondence to Takashi Ito .

Editor information

Editors and Affiliations

  1. Laboratory for Epigenetics and Environment, Centre National de Recherche en Génomique Humaine, CEA—Institut de Biologie Francois Jacob, Evry, France

    Jörg Tost

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Miura, F., Ito, T. (2018). Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing. In: Tost, J. (eds) DNA Methylation Protocols. Methods in Molecular Biology, vol 1708. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7481-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7481-8_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7479-5

  • Online ISBN: 978-1-4939-7481-8

  • eBook Packages: Springer Protocols

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