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Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells

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B Cell Receptor Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1707))

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Abstract

B lymphocytes and their differentiated daughter cells are charged with responding to invading pathogens and producing protective antibodies against these pathogens. The physiology of B cells is intimately connected with the function of the B cell antigen receptor (BCR). Upon activation of BCR, transmembrane signals are generated, and several downstream pathways are activated, which provide a primary directive for the cell’s subsequent response. mTOR is a serine/threonine kinase that controls cell proliferation and metabolism in response to a diverse range of extracellular stimuli. The activation of mTOR signaling downstream of PI3K/Akt activity by B cell receptor (BCR) engagement has been generally assumed to be essential for B cell responses. This chapter seeks to present two protocols to evaluate mTOR activity in B cells bearing BCR specific to 4-hydroxy-3-nitrophenylacetyl (NP)-hapten.

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Acknowledgments

The work was supported by National Basic Research Program of China (973 program, 2013CB531500, to L.Y.), The National Key Research and Development Program of China (2016YFA0502202 to L.Y.).

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Authors and Affiliations

  1. Institute of Immunology, Third Military Medical University, Chongqing, 400038, China

    Qizhao Huang, Haoqiang Wang, Lifan Xu, Jianjun Hu, Pengcheng Wang, Yiding Li & Lilin Ye

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  1. Qizhao Huang
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  2. Haoqiang Wang
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  3. Lifan Xu
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  4. Jianjun Hu
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  5. Pengcheng Wang
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  7. Lilin Ye
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Corresponding author

Correspondence to Lilin Ye .

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Editors and Affiliations

  1. Department of Pathogen Biology, Huazhong University of Science and Technology, Huazhong, China

    Chaohong Liu

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Huang, Q. et al. (2018). Flow Cytometry Analysis of mTOR Signaling in Antigen-Specific B Cells. In: Liu, C. (eds) B Cell Receptor Signaling. Methods in Molecular Biology, vol 1707. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7474-0_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7474-0_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7473-3

  • Online ISBN: 978-1-4939-7474-0

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