A Fluorescence-Based Assay for Identification of Bacterial Topoisomerase I Poisons
Bacterial Topoisomerase I is a potential target for the identification of novel topoisomerase poison inhibitors that could provide leads for a new class of antibacterial compounds. Here we describe in detail a fluorescence-based cleavage assay that is successfully used in HTS for the discovery of bacterial topoisomerase Ι poisons.
Key wordsBacterial topoisomerase I Topoisomerase I poisons Fluorescence-based assay High-throughput screening
We thank the staff at NERCE/NSRB for access to screening libraries and HTS facilities, assistance in assay setup, data processing and analysis. This work was supported by funding from TB Alliance and National Institutes of Health grants R21NS067592, R01AI069313 to YT.
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