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NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE

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Bacterial Multidrug Exporters

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1700))

Abstract

Multidrug exporters are a class of membrane proteins that remove antibiotics from the cytoplasm of bacteria and in the process confer multidrug resistance to the organism. This chapter outlines the sample preparation and optimization of oriented solid-state NMR experiments applied to the study of structure and dynamics for the model transporter EmrE from the small multidrug resistance (SMR) family.

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Acknowledgments

This work was supported by NIH (R01AI108889) and NSF (MCB1506420). M.L. acknowledges support from a Margaret-Strauss Kramer Fellowship.

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Authors and Affiliations

  1. Department of Chemistry, New York University, 100 Washington Square East, New York, NY, 10003, USA

    Maureen Leninger & Nathaniel J. Traaseth

Authors
  1. Maureen Leninger
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  2. Nathaniel J. Traaseth
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Corresponding author

Correspondence to Nathaniel J. Traaseth .

Editor information

Editors and Affiliations

  1. Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, Japan

    Akihito Yamaguchi

  2. Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan

    Kunihiko Nishino

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Leninger, M., Traaseth, N.J. (2018). NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE. In: Yamaguchi, A., Nishino, K. (eds) Bacterial Multidrug Exporters. Methods in Molecular Biology, vol 1700. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7454-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7454-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7452-8

  • Online ISBN: 978-1-4939-7454-2

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