Abstract
Circulating microRNAs are increasingly being used as noninvasive prognostic and predictive biomarkers for various types of cancer. We describe a method that uses real-time quantitative PCR (qPCR) for establishing a signature plasma microRNA profile that can distinguish patients with osteosarcoma from healthy control samples.
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Acknowledgments
This work was funded through the generous support of a St. Baldrick’s Foundation Fellowship, the Amschwand Sarcoma Cancer Foundation’s Dr. Stephan Fadem Fellowship, and a Conquer Cancer Foundation of ASCO Young Investigator Award, supported by WWWW Foundation (Quad W). Research is also supported by the Chair’s Grant U10 CA98543 and Human Specimen Banking Grant U24 CA114766 of the Children’s Oncology Group from the National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. Additional support for research is provided by a grant from the WWWW (QuadW) Foundation, Inc. (www.QuadW.org) to the Children’s Oncology Group. Any opinions, findings, and conclusions expressed in this material are those of the authors and do not necessarily reflect those of the American Society of Clinical Oncology®, the Conquer Cancer Foundation, or the WWWW Foundation (Quad W).
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Allen-Rhoades, W., Yustein, J.T. (2018). Detection of Plasma MicroRNA Signature in Osteosarcoma Patients. In: Wu, W. (eds) MicroRNA and Cancer. Methods in Molecular Biology, vol 1699. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7435-1_9
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DOI: https://doi.org/10.1007/978-1-4939-7435-1_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7433-7
Online ISBN: 978-1-4939-7435-1
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