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Angiogenesis Analysis by In Vitro Coculture Assays in Transwell Chambers in Ovarian Cancer

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MicroRNA and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1699))

Abstract

Angiogenesis is an important biological process in tumor growth and metastasis of tumor cells, and it has been associated with poor clinical outcomes in ovarian cancer. In vitro assays are useful tools for understanding the complex mechanisms of angiogenesis under a variety of conditions. Capillary-like formation and transwell migration assays are two of the most common techniques used in angiogenesis research. Here, we show an easy coculture model to study the role of microRNAs on angiogenesis that combines tube formation and cell migration assays. Recently, we reported that miR-204 is repressed in breast cancer and restoration in cancer cell lines results in angiogenesis inhibition. Here, we restored the expression of miR-204 by transfection of precursor molecule in the tumorigenic SKOV3 ovarian cancer cell line, and analyzed the effects in cell migration, invasion, and tube formation of endothelial cells using matrigel-coated transwell chambers.

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Authors and Affiliations

  1. Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, San Lorenzo 290, Del Valle Sur, 03100, Benito Juarze, CDMX, Mexico

    Ali Flores-Pérez, Raquel Echavarria, Elizbeth Álvarez-Sánchez & César López-Camarillo

  2. Laboratorio de Medicina Translacional, Instituto Nacional de Cancerología, Sección XVI, Niño Jesús, 14080, Tlalpan, CDMX, Mexico

    Dolores Gallardo Rincón & Erika Ruiz-García

  3. Programa en Biomedicina Molecular y Red de Biotecnología, Escuela Nacional de Medicina y Homeopatía, Instituto Politécnico Nacional, Av. Guillermo Massieu Helguera 239, La Escalera, 07320, Ciudad de México, CDMX, Mexico

    Laurence A. Marchat

Authors
  1. Ali Flores-Pérez
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  2. Dolores Gallardo Rincón
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  3. Erika Ruiz-García
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  4. Raquel Echavarria
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  5. Laurence A. Marchat
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  6. Elizbeth Álvarez-Sánchez
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  7. César López-Camarillo
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Corresponding author

Correspondence to César López-Camarillo .

Editor information

Editors and Affiliations

  1. Department of Medicine, Helen Diller Family Comprehensive Cancer Center, University of California in San Francisco, San Francisco, California, USA

    Wei Wu

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Flores-Pérez, A. et al. (2018). Angiogenesis Analysis by In Vitro Coculture Assays in Transwell Chambers in Ovarian Cancer. In: Wu, W. (eds) MicroRNA and Cancer. Methods in Molecular Biology, vol 1699. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7435-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7435-1_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7433-7

  • Online ISBN: 978-1-4939-7435-1

  • eBook Packages: Springer Protocols

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