Erythropoiesis pp 103-115 | Cite as

Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1698)

Abstract

Over the last decades several mouse models of human hemoglobin disorders have been and continue to be generated. This chapter aims at describing various approaches to evaluate the global red blood cell properties in mouse models of human hemoglobin disorders, in particular thalassemia and sickle cell disease. Analysis of erythroid parameters provides insights into the RBC physiologic or pathophysiologic status. Mice expressing both murine and human globin genes can be investigated using adapted protocols provided herein.

Key words

Hemoglobin Globin chain Urea-triton polyacrylamide gel electrophoresis Cellulose acetate electrophoresis Smear 

Notes

Acknowledgment

This work was supported by Canadian Blood Services and Canadian Institute for Health Research grants to M.T.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Molecular Genetics and Development, Institut de Recherches Cliniques de Montréal, Faculté de MédecineUniversité de MontréalMontrealCanada

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