Abstract
Genome editing to introduce specific mutations or to knock out genes in model cell systems has become an efficient platform for research in the fields of molecular biology, genetics, and cell biology. With recent rapid improvements in genome editing techniques, bench-top manipulation of the genome in cell culture has become progressively easier. The application of this knowledge to erythroid cell culture systems now allows the rapid analysis of the downstream effects of virtually any engineered gene disruption or modification in cell systems. Here, we describe a CRISPR/Cas9-based approach to making genomic modifications in erythroid lineage cells which we have successfully used in both murine (MEL) and human (K562) erythroleukaemia immortalized cell lines.
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Acknowledgment
This work has been supported by funding from the Australian Research Council and the National Health and Medical Research Council to M.C.
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Yik, J.J., Crossley, M., Quinlan, K.G.R. (2018). Genome Editing of Erythroid Cell Culture Model Systems. In: Lloyd, J. (eds) Erythropoiesis. Methods in Molecular Biology, vol 1698. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7428-3_15
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DOI: https://doi.org/10.1007/978-1-4939-7428-3_15
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