Erythropoiesis pp 245-257 | Cite as

Genome Editing of Erythroid Cell Culture Model Systems

  • Jinfen J. Yik
  • Merlin Crossley
  • Kate G. R. Quinlan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1698)

Abstract

Genome editing to introduce specific mutations or to knock out genes in model cell systems has become an efficient platform for research in the fields of molecular biology, genetics, and cell biology. With recent rapid improvements in genome editing techniques, bench-top manipulation of the genome in cell culture has become progressively easier. The application of this knowledge to erythroid cell culture systems now allows the rapid analysis of the downstream effects of virtually any engineered gene disruption or modification in cell systems. Here, we describe a CRISPR/Cas9-based approach to making genomic modifications in erythroid lineage cells which we have successfully used in both murine (MEL) and human (K562) erythroleukaemia immortalized cell lines.

Key words

Genome editing Genome engineering CRISPR/Cas9 Erythrocyte MEL K562 

Notes

Acknowledgment

This work has been supported by funding from the Australian Research Council and the National Health and Medical Research Council to M.C.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  • Jinfen J. Yik
    • 1
  • Merlin Crossley
    • 1
  • Kate G. R. Quinlan
    • 1
  1. 1.School of Biotechnology and Biomolecular SciencesUniversity of New South WalesSydneyAustralia

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