Abstract
Chromatin immunoprecipitation (ChIP) allows determination of the locations to which a select protein is bound in chromatin. Chemical crosslinking of DNA and protein with bi-functional reagents such as formaldehyde and precipitation of the protein with a specific antibody permit PCR amplification (ChIP) or sequencing (ChIP-seq) to identify the bound sites. Here, we present methodology for these approaches that are widely applicable to erythroid cell lines, progenitor cells, and tissues.
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Acknowledgments
This work was supported by the Intramural Program of the National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (DK015508 to AD).
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Krivega, I., Dean, A. (2018). Chromatin Immunoprecipitation (ChIP) with Erythroid Samples. In: Lloyd, J. (eds) Erythropoiesis. Methods in Molecular Biology, vol 1698. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7428-3_13
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DOI: https://doi.org/10.1007/978-1-4939-7428-3_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7427-6
Online ISBN: 978-1-4939-7428-3
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