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Burkholderia Sp. Strain BBK_9: A Potent Agent for Propiconazole Degradation

Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Propiconazole, a leading triazoles fungicide, used to protect the standing plants from fungal diseases. The residues of this fungicide are known to persist in agricultural soil for longer duration. Therefore, in the present study, a newly isolated potent bacterium Burkholderia sp. strain BBK_9 was opted for the degradation of propiconazole (20 μg/mL) by immobilization process. The effect of propiconazole (10, 20, 30 μg/mL) on nucleic acids (DNA and RNA), glucose, and stress enzymes of BBK_9 strain was investigated. Furthermore, the induction of different proteins during the degradation of propiconazole was elucidated by the SDS PAGE analysis. Molecular functional dissimilarity in BBK_9 strain when exposed propiconazole was examined by the Fourier Transmission Infrared Spectroscopy (FTIR). The results indicate that immobilized cells of BBK_9 strain utilized propiconazole up to 19.2 μg/mL after 96 h at 30 °C and pH 7. Moreover, the highest concentration of propiconazole (30 μg/mL) was effected BBK_9 strain, and contents of DNA, RNA, and glucose were significantly decreased, additionally, stress enzymes were significantly increased at 30 μg/mL of propiconazole. The bacterial cells were aided by the secretion major proteins between 43 and 29 kDa in the degradation process. Production of molecular functional groups was founded more in the propiconazole treated strain than in the untreated bacterial strain. These outcomes revealed that Burkholderia sp. BBK_9 strain was found to be promising agent for remediation of pollutants; meanwhile, the excess of concentration will also be harmful for the soil health and microbial community.

Key words

Propiconazole Biodegradation Immobilization Nucleic acids SDS PAGE FTIR 

Notes

Acknowledgment

The authors are thankful to the Department of Biotechnology (DBT), Government of India, Delhi for proving the Bioinformatics infra-structure facility and IPLS programme. Authors also thankful to the UGC-UPE fellowships and Post Graduate Department of Studies in Microbiology and Biotechnology, Karnatak University Dharwad for providing the laboratory facilities.

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© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Studies and Research in Microbiology and BiotechnologyKarnatak UniversityDharwadIndia
  2. 2.Davangere UniversityShivgangotri, DavangereIndia

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