Bioremediation of Methomyl by Escherichia coli

Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Methomyl belonging to N-methyl carbamate group of insecticide classified as a toxic, hazardous and restricted use pesticide by the World Health Organization (WHO), European Commission (EC), and Environmental Protection Act (EPA), is the most commonly used pesticide on fruits and vegetables. The present study is, therefore, aimed to study the potentiality of Escherichia coli in the degradation of methomyl and further determine the role of plasmid in bioremediation. The organism was inoculated in a synthetic medium containing methomyl (10−3–10−7 M) for 24, 48, 72, and 96 h and subjected to HPLC analysis where methomyl degradation (%) was observed. The present study on HPLC analysis at regular intervals of 24 h of Escherichia coli in the synthetic medium containing methomyl (10−3–10−7 M) incubated for 96 h revealed that there was a significant decrease in the methomyl content in all the treated groups when compared with the initial control, thus offering a promising strategy toward bioremediation. The plasmid isolation and curing study confirmed that the genes of both the plasmid and the main chromosome of Escherichia coli are involved in the methomyl degradation. The evolution and spread of pesticide-degrading genes may have a beneficial effect by removing toxic pollutants from the environment. Such organisms can be a source of resistance genes for cloning purpose which have potential use in biotechnology such as the manufacture of biosensors and bioremediation processes.

Key words

Bioremediation Methomyl Escherichia coli Plasmid 

Notes

Acknowledgments

The authors are grateful to the Post Graduate Department of Studies in Microbiology and Biotechnology, Karnatak University Dharwad, Bioinformatics Infrastructure facility centre, DBT, New Delhi and IPLS, DST for providing the necessary facilities.

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Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.P.G. Department of Studies in Biotechnology and MicrobiologyKarnatak UniversityDharwadIndia
  2. 2.Davangere UniversityShivgangotri, DavangereIndia

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