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Whole Effluent Toxicity Assessment of Industrial Effluents

  • Takashi Kusui
  • Yasuyuki Itatsu
  • Jun Jin
Protocol
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Direct discharge of industrial effluents into aquatic ecosystems continues to be an important area of concern because of potential ecotoxic impact on biota in receiving water. Biological tests with aquatic organisms have the capacity to respond to chemicals and quantify their effects even if they are present as mixtures or are unidentified. To compensate for the shortcomings of traditional effluent regulation, whole-effluent toxicity (WET) testing was introduced in the USA, Canada, European countries, and South Korea. Additionally, to reduce the toxicity levels detected in effluents, a procedure called toxicity reduction evaluation (TRE) was proposed. Based on the lessons learnt in the implementation of WET testing in these countries, this approach, involving chronic assays, was suggested for introduction in Japan. Here, we present a short review on the bioassay-based regulations and biological methods prevalent in these countries. Furthermore, we introduce two case studies from Japan. The first study reports on short-chronic assays used as WET tests whereas the second discusses TRE and the application of toxicity identification evaluation (TIE) to identify causative factors, employing a combination of biological tests and physiochemical manipulations. We also discuss simple and rapid bioassays for routine monitoring of effluent toxicity.

Key words

Whole-effluent toxicity (WET) Bioassay Industrial effluent Toxicity reduction evaluation (TRE) Toxicity identification evaluation (TIE) 

Notes

Acknowledgments

The authors thank Dr. Christian Blaise for helpful comments on the manuscript.

Glossary

ECx

x percentage of effective concentration

ICx

x percentage of inhibitory concentration

NOEC

No observed effect concentration

TIE

Toxicity identification evaluation

TRE

Toxicity reduction evaluation

TUc

Chronic toxicity unit

References

  1. 1.
    USEPA (1991) Technical support document for water quality-based toxics control. Washington, D.C. EPA-505-2-90-001Google Scholar
  2. 2.
    Power EA, Boumphrey RS (2004) International trends in bioassay use for effluent management. Ecotoxicology 13(5):377–398CrossRefPubMedGoogle Scholar
  3. 3.
    Tatarazako N (2006) Bioassays for environmental water: a perspective in whole effluent toxicity. J Jpn Soc Water Environ 29(8):426–432. (in Japanese)Google Scholar
  4. 4.
    Dorn PB, van Compernolle R (1995) Effluents. In: Rand GM (ed) Fundamentals of aquatic toxicology, 2nd edn. Tayler & Francis, WashingtonGoogle Scholar
  5. 5.
    Chapman PM (2000) Whole effluent toxicity testing—usefulness, level of protection, and risk assessment. Environ Toxicol Chem 19(1):3–13Google Scholar
  6. 6.
    USEPA (1991) Methods for aquatic toxicity identification evaluations: phase I toxicity characterization procedures. EPA-600-6-91-003Google Scholar
  7. 7.
    USEPA (1993) Methods for aquatic toxicity identification evaluations: phase II toxicity identification procedures for samples exhibiting acute and chronic toxicity. EPA-600-R-92-080Google Scholar
  8. 8.
    USEPA (1993) Methods for aquatic toxicity identification evaluations: phase III toxicity confirmation procedures for samples exhibiting acute and chronic toxicity. EPA-600-R-92-081Google Scholar
  9. 9.
    Ministry of the Environment and National Institute for Environmental Studies (2012) Proceedings of seminar on effluent management approaches using biological response in Foreign CountriesGoogle Scholar
  10. 10.
    Kusui T, Blaise C (2003) Ecotoxicological assessment of Japanese industrial effluents using a battery of small-scale toxicity tests. In: Rao SS (ed) Impact assessment of hazardous aquatic contaminants. Lewis Publisher, Boca RatonGoogle Scholar
  11. 11.
    Kusui T, Takata Y, Itatsu Y, Zha J (2014) Whole effluent toxicity assessment of industrial effluents in Toyama Prefecture with a battery of short-term chronic bioassays. J Water Environ Technol 12:55–63CrossRefGoogle Scholar
  12. 12.
    Itatsu Y, Takano T, Jin J, Fukutomi M, Kusui T (2015) Ecotoxicological assessment of industrial effluents: toxicity characterization and impact on receiving water. J Environ Chem 25(1):19–26. (in Japanese)CrossRefGoogle Scholar
  13. 13.
    OECD (2006) Guidelines for the testing of chemicals no.201, Freshwater Alga and Cyanobacteria, Growth Inhibition Test, Paris, FranceGoogle Scholar
  14. 14.
    Environment Canada (2007) Biological test method: test of reproduction and survival using the cladoceran Ceriodaphnia dubia, EPS 1/RM/21 Second editionGoogle Scholar
  15. 15.
    OECD (1998) Guideline for testing of chemicals no.212, Fish, short-term toxicity test on embryo and sac-fry stages, Paris, FranceGoogle Scholar
  16. 16.
    Keithly J, Brooker JA, DeForest DK, Wu BK, Brix KV (2004) Acute and chronic toxicity of nickel to a cladoceran (Ceriodaphnia dubia) and an amphipod (Hyalella azteca). Environ Toxicol Chem 23:691–696CrossRefPubMedGoogle Scholar
  17. 17.
    Emerson K, Russo CR, Lund RE, Thurston RV (1975) Aqueous ammonia equilibrium calculations: effect of pH and temperature. J Fish Res Board Can 32:2379–2383CrossRefGoogle Scholar
  18. 18.
    Johnson C G (1995) Effects of pH and hardness on acute and chronic toxicity of un-ionized ammonia to Ceriodaphnia dubia, MSThesis, University of Wisconsin, Stevens Point, WI.69pGoogle Scholar
  19. 19.
    Ford LD (ed) (1998) Toxicity reduction: evaluation and control. Techonomic Publishing Company Inc., PennsylvaniaGoogle Scholar
  20. 20.
    Norverg-King TJ, Ausley LW, Burton DT, Goodfellow WL, Miller JI, Waller WT (2005) Toxicity reduction and toxicity identification evaluations for effluents, ambient waters, and other aqueous media. Soc Environ Toxicol ChemGoogle Scholar
  21. 21.
    Johnson TM (2013) Bacteria in ecotoxicology: microtox basic. In: Ferard J-F, Blaise C (eds) Encyclopedia of aquatic ecotoxicology Vol.1. SpringerGoogle Scholar
  22. 22.
    Persoone P (1991) Cyst-based toxicity test. I.A promising new tool for rapid and cost-effective toxicity screening of chemicals and effluents. Z Angew Zool 78:235–241Google Scholar
  23. 23.
    Katsumata M, Koike T, Kazumura K, Takeuchi A, Sugaya Y (2009) Utility of delayed fluorescence as endpoint for rapid estimation of effect concentration on the green alga Pseudokirchenriella subcapitata. Bull Environ Contam Toxicol 83:484–487CrossRefPubMedGoogle Scholar
  24. 24.
    Takeuchi A, Katsumata M, Koike T, Takata Y, Itatsu Y, Kusui T (2014) Comparison of the conventional algal growth inhibition tests using cell counting and algal bioassay using delayed fluorescence: application to industrial effluents. J Water Environ Technol 12:367–377CrossRefGoogle Scholar
  25. 25.
    Persoone G, Janssen C, Coen WD (2000) New microbiotests for routine toxicity screening and biomonitoring. Kluwer Academic/Plenu Publisher, New YorkCrossRefGoogle Scholar
  26. 26.
    Blaise C, Ferard J-F (2005) Small-scale freshwater toxicity investigations vol.1: toxicity test methods. Springer, the NetherlandCrossRefGoogle Scholar
  27. 27.
    Strahle U et al (2012) Zebrafish embryos as an alternative to animal experiments—a commentary on the definition of the onset of protected life stages in animal welfare regulations. Reprod Toxicol 33(2):128–132CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2018

Authors and Affiliations

  1. 1.Department of Environmental and Civil EngineeringToyama Prefectural UniversityImizuJapan

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