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Biodegradation of Dyes Intermediates and HPLC Method for Their Estimation

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Toxicity and Biodegradation Testing

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

The industrial synthesis of pigments and dyes generates hazardous chemicals. These chemicals are released into water, soil, and environment and regarded as a major source of environmental pollution. H acid is also an important dye intermediate. In this study, the focus was on the bacterial degradation of H acid (1-amino-8-naphthol-3,6-disulfonic acid) of different concentrations and its degradation estimation method. The biodegradation was identified by the help of IR, UV-Visible spectrophotometer, and high-performance liquid chromatography (HPLC). A method was developed for the estimation of percent biodegradation of H acid with the help of HPLC. The developed method was applicable on standard as well as biodegraded products and on other compounds with the sulfonic acid group. It was found that H acid of concentration 300 ppm and below can be completely degraded with Bacillus subtilis. However, over 300 ppm, the bacterial degradation did not work efficiently.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Government College University, Katchery Road, Lahore, 54000, Pakistan

    Rana Rashad Mahmood Khan, Saira Saeed & Ahmad Adnan

Authors
  1. Rana Rashad Mahmood Khan
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  2. Saira Saeed
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  3. Ahmad Adnan
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Corresponding author

Correspondence to Rana Rashad Mahmood Khan .

Editor information

Editors and Affiliations

  1. Biosciences Institute, São Paulo State University (UNESP), Rio Claro, São Paulo, Brazil

    Ederio Dino Bidoia

  2. Biosciences Institute, São Paulo State University (UNESP), Rio Claro, São Paulo, Brazil

    Renato Nallin Montagnolli

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Khan, R.M., Saeed, S., Adnan, A. (2018). Biodegradation of Dyes Intermediates and HPLC Method for Their Estimation. In: Bidoia, E., Montagnolli, R. (eds) Toxicity and Biodegradation Testing. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7425-2_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7425-2_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7424-5

  • Online ISBN: 978-1-4939-7425-2

  • eBook Packages: Springer Protocols

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