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Glaucoma pp 171–185Cite as

Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1695))

Abstract

Whether to stage degeneration or investigate early pathology in glaucoma, examination of axonal structure and function is essential. There are a wide variety of methods available to investigators using animal models of glaucoma, with varying utilities depending on the questions asked. Here, we describe the use of anterograde neuronal tract tracing using cholera toxin B (CTB) for the determination of axon transport integrity of the retinofugal projection. This method reveals the structure of the retinal axons as well as the functional integrity of anterograde transport systems.

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Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Northeast Ohio Medical University, RGE-135, 4209 St. Rt. 44, Rootstown, OH, 44272, USA

    Samuel D. Crish

  2. Department of Anatomy and Neurobiology, Northeast Ohio Medical University, Rootstown, OH, 44272, USA

    Brett R. Schofield

Authors
  1. Samuel D. Crish
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  2. Brett R. Schofield
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Corresponding author

Correspondence to Samuel D. Crish .

Editor information

Editors and Affiliations

  1. Department of Ophthalmology, Massachusetts Eye and Ear Infirmary/Schepens Eye, Research Institute, Harvard Medical School, Boston, Massachusetts, USA

    Tatjana C. Jakobs

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Crish, S.D., Schofield, B.R. (2018). Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma. In: Jakobs, T. (eds) Glaucoma. Methods in Molecular Biology, vol 1695. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7407-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7407-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7406-1

  • Online ISBN: 978-1-4939-7407-8

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