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Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion

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Glaucoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1695))

Abstract

Mice and rats are being increasingly used in glaucoma research and much useful data have been generated from them. One aspect of using these animals for this purpose involves assessment of aqueous humor dynamics. Several techniques have been described in the literature for the determination of one or more of these parameters in rodents, in both living animals and eyes perfused ex vivo. Here, we describe the practical details for a technique for the determination of all principal parameters of aqueous humor dynamics (intraocular pressure (IOP), aqueous humor formation rate (Fin), uveoscleral outflow rate (Fu), aqueous outflow facility (C), and episcleral venous pressure (Pe)) in the living rat and mouse eye, in a single experimental session.

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References

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Authors and Affiliations

  1. North Texas Eye Research Institute, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX, 76107, USA

    J. Cameron Millar, Tien N. Phan & Iok-Hou Pang

  2. Department of Pharmaceutical Sciences, College of Pharmacy, University of North Texas Health Science Center, 3500 Camp Bowie Boulevard, Fort Worth, TX, 76107, USA

    Iok-Hou Pang

Authors
  1. J. Cameron Millar
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  2. Tien N. Phan
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  3. Iok-Hou Pang
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Corresponding author

Correspondence to J. Cameron Millar .

Editor information

Editors and Affiliations

  1. Department of Ophthalmology, Massachusetts Eye and Ear Infirmary/Schepens Eye, Research Institute, Harvard Medical School, Boston, Massachusetts, USA

    Tatjana C. Jakobs

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Millar, J.C., Phan, T.N., Pang, IH. (2018). Assessment of Aqueous Humor Dynamics in the Rodent by Constant Flow Infusion. In: Jakobs, T. (eds) Glaucoma. Methods in Molecular Biology, vol 1695. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7407-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7407-8_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7406-1

  • Online ISBN: 978-1-4939-7407-8

  • eBook Packages: Springer Protocols

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