Abstract
This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.
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Trzeciecka, A. et al. (2018). Shotgun Sphingolipid Analysis of Human Aqueous Humor. In: Jakobs, T. (eds) Glaucoma. Methods in Molecular Biology, vol 1695. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7407-8_10
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DOI: https://doi.org/10.1007/978-1-4939-7407-8_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7407-8
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