Skip to main content
SpringerLink
Log in

Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods

  • Protocol
  • First Online:
Cancer Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1692))

Abstract

Cancer stem cells (CSCs) are rare immortal cells within a tumor that can self-renew and drive tumorigenesis. CSCs play a pivotal role in the tumor development, progression and relapse, as well as in the resistance of anticancer therapy. Different tools could help in the analysis of CSCs, especially Immunohistochemistry (IHC) represents a useful technique able to identify several specific CSC markers. The main aims of this chapter are the description of the explain immunohistochemical methods used in the characterization of CSCs. Furthermore, focus on the most common troubleshooting in CSCs IHC is provided, especially the pitfalls of the CSCs markers IHC on tissue microarrays.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Clarke MF, Dick JE, Dirks PB et al (2006) Cancer stem cells-perspectives on current status and future directions: AACR workshop on cancer stem cells. Cancer Res 66:9339–9344

    Article  CAS  PubMed  Google Scholar 

  2. Krause M, Dubrovska A, Linge A, Baumann M (2016) Cancer stem cells: radioresistance, prediction of radiotherapy out come and specific targets for combined treatments. Adv Drug Deliv Rev 109:63–73

    Article  PubMed  Google Scholar 

  3. Vinogradov S, Wei X (2012) Cancer stem cells and drug resistance: the potential of nanomedicine. Nanomedicine (Lond) 7:597–615. doi:10.2217/nnm.12.22

    Article  CAS  Google Scholar 

  4. Baumann M, Krause M, Thames H et al (2009) Cancer stem cells and radiotherapy. Int J Radiat Biol 85:391–402

    Article  CAS  PubMed  Google Scholar 

  5. Baumann M, Krause M, Hill R (2008) Exploring the role of cancer stem cells in radioresistance. Nat Rev Cancer 8:545–554

    Article  CAS  PubMed  Google Scholar 

  6. Cancer Stem Cell Markers. Available: https://www.rndsystems.com/research-area/cancer-stem-cell-markers

  7. Ramos-Vara JA, Beissenherz ME (2000) Optimization of immunohistochemical methods using two different antigen retriva methods on formalin-fixed, paraffin-embedded tissues: experience with 63 markers. J Vet Diagn Investig 12:307–311

    Article  CAS  Google Scholar 

  8. Ramos-Vara JA (2005) Technical aspects of immunohistochemistry. Vet Pathol 42:405–426

    Article  CAS  PubMed  Google Scholar 

  9. Van der Loos CM (1999) Immunoenzyme multiple staining methods. Bios Scientific Publishers, Oxford, UK

    Google Scholar 

  10. Atkins D, Reiffen KA, Tegtmeier CL et al (2004) Immunohistochemical detection of EGFR in paraffin-embedded tumor tissues: variation in staining intensity due to choice of fixative and storage time of tissue sections. J Histochem Cytochem 52:893–901

    Article  CAS  PubMed  Google Scholar 

  11. Fergenbaum JH, Gracia-Closas M, Hewitt SM et al (2004) Loss of antigenicity in stored sections of breast cancer tissue microarrays. Cancer Epidemiol Biomark Prev 13:667–672

    CAS  Google Scholar 

  12. Mirlacher M, Kasper M, Storz M et al (2004) Influence of slide aging on results of translational research studies using immunohistochemistry. Mod Pathol 17:1414–1420

    Article  PubMed  Google Scholar 

  13. Blind C, Koepenik A, Pacyna-Gengelbach M et al (2008) Antigenicity testing by immunohistochemistry after tissue oxidation. J Clin Pathol 61:79–83

    Article  CAS  PubMed  Google Scholar 

  14. Wood GS, Warnke R (1981) Suppression of endogenous avidin-binding activity in tissues and its relevance to biotinavidin detection systems. J Histochem Cytochem 29:1196–1204

    Article  CAS  PubMed  Google Scholar 

  15. Henwood AF (2005) Effect of slide drying at 80°C on immunohistochemistry. J Histotechnol 28:45–46

    Article  Google Scholar 

  16. Malik NJ, Daymon ME (1982) Improved double immunoenzyme labeling using alkaline phosphatase and horseradish peroxidase. J Clin Pathol 35:1092–1094

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Cantile M, Collina F, Scognamiglio G et al (2013) Inadequacy of tissue microarrays for the immunohistochemical detection of cncer stem cells in solid tumors: a viewpoint. Expert Rev Anticancer Ther 13:1139–1141

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pathology Unit, University of Campania “Luigi Vanvitelli”, Via Luciano Armanni, 20, 80138, Naples, Italy

    Martina Intartaglia, Rosalaura Sabetta, Monica Gargiulo, Federica Zito Marino & Renato Franco

  2. Monoclonal Antibody Unit, Spanish National Cancer Research Center, Madrid, Spain

    Giovanna Roncador

Authors
  1. Martina Intartaglia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rosalaura Sabetta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Monica Gargiulo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Giovanna Roncador
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Federica Zito Marino
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Renato Franco
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Renato Franco .

Editor information

Editors and Affiliations

  1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio

  2. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Vincenzo Desiderio

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Intartaglia, M., Sabetta, R., Gargiulo, M., Roncador, G., Marino, F.Z., Franco, R. (2018). Immunohistochemistry for Cancer Stem Cells Detection: Principles and Methods. In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7401-6_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7400-9

  • Online ISBN: 978-1-4939-7401-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation