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Cancer Stem Cells pp 179–193Cite as

Histones Acetylation and Cancer Stem Cells (CSCs)

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1692))

Abstract

Chromatin decondensation is a key mechanism that guarantees gene transcription and repair of the genome, regulated mainly by the acetylation of histones. Emerging evidence has pointed out to histones as a new controlling mechanism of stem cell maintenance and fate. In this chapter, we will focus on the methods used to enrich tumor cell lines for cancer stem cells, and in the methods to identify the status of the histone acetylation in cancer cells and stem cells using immunofluorescence, invasion, and adhesion assays and identification of nuclear size.

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Author information

Authors and Affiliations

  1. Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil

    Vivian Petersen Wagner & Manoela Domingues Martins

  2. Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, 48109-1078, USA

    Rogerio Moraes Castilho

Authors
  1. Vivian Petersen Wagner
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  2. Manoela Domingues Martins
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  3. Rogerio Moraes Castilho
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Corresponding author

Correspondence to Rogerio Moraes Castilho .

Editor information

Editors and Affiliations

  1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio

  2. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Vincenzo Desiderio

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Wagner, V.P., Martins, M.D., Castilho, R.M. (2018). Histones Acetylation and Cancer Stem Cells (CSCs). In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7401-6_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7400-9

  • Online ISBN: 978-1-4939-7401-6

  • eBook Packages: Springer Protocols

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