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Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML)

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Cancer Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1692))

Abstract

Epithelial Mesenchymal Transition (EMT) is a key event in cancer progression. During this event, epithelial cancer cells undergo molecular and cellular changes leading to their trans-differentiation into mesenchymal cancer cells that are capable of migration, invasion, and metastasis to other tissues and organs. Here, we present a method for in vitro induction of EMT in prostate cancer cell lines using lentiviral expression of a PMLI isoform mutant construct.

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Acknowledgment

This work was supported by the John and Lucille van Geest Foundation, and the John van Geest Cancer Research Centre, Nottingham Trent University.

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Authors and Affiliations

  1. The John van Geest Cancer Research Centre, Nottingham Trent University, Clifton lane, Nottingham, NG11 8NS, UK

    Anna Di Biase, Amanda K. Miles & Tarik Regad

Authors
  1. Anna Di Biase
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  2. Amanda K. Miles
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  3. Tarik Regad
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Corresponding author

Correspondence to Tarik Regad .

Editor information

Editors and Affiliations

  1. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Gianpaolo Papaccio

  2. Department of Experimental Medicine, University of Campania “Luigi Vanvitelli”, Naples, Italy

    Vincenzo Desiderio

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Di Biase, A., Miles, A.K., Regad, T. (2018). Generation of In Vitro Model of Epithelial Mesenchymal Transition (EMT) Via the Expression of a Cytoplasmic Mutant Form of Promylocytic Leukemia Protein (PML). In: Papaccio, G., Desiderio, V. (eds) Cancer Stem Cells. Methods in Molecular Biology, vol 1692. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7401-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7401-6_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7400-9

  • Online ISBN: 978-1-4939-7401-6

  • eBook Packages: Springer Protocols

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