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Modulation of Protein Synthesis by Polyamines in Mammalian Cells

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Book cover Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Polyamines exist mainly as RNA–polyamine complexes in cells. Thus, we looked for proteins whose synthesis is enhanced by polyamines at the level of translation in mammalian cells. Here, we describe how synthesis of Cct2 (T-complex protein 1, β-subunit, a chaperonin assisting in the folding actin, tubulin, and several other proteins) and eEF1A (one of the elongation factors of protein synthesis) is stimulated by polyamines at the level of translation. Polyamines stimulated Cct2 synthesis through the stimulation of ribosome shunting during 5′-processive scanning of 40S ribosomal subunits from the m7G-cap to the initiation codon AUG, and eEF1A synthesis through the structural change of the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA.

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Acknowledgments

We are grateful to Dr. A.J. Michael for critical reading of the manuscript prior to submission.

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Authors and Affiliations

  1. Faculty of Pharmacy, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan

    Keiko Kashiwagi & Yusuke Terui

  2. Amine Pharma Research Institute, Innovation Plaza at Chiba University, 1-8-15 Inohana, Chuo-ku, Chiba, 260-0856, Japan

    Kazuei Igarashi

  3. Graduate School of Pharmaceutical Sciences, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8675, Japan

    Kazuei Igarashi

Authors
  1. Keiko Kashiwagi
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  2. Yusuke Terui
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  3. Kazuei Igarashi
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Corresponding author

Correspondence to Keiko Kashiwagi .

Editor information

Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Kashiwagi, K., Terui, Y., Igarashi, K. (2018). Modulation of Protein Synthesis by Polyamines in Mammalian Cells. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_27

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

  • eBook Packages: Springer Protocols

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