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Techniques Used for Functional Characterization of Polyamine Transporters

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Transport systems are key processes in every living organism: they allow the entry of all essential nutrients into the cell and its compartments and regulate the intracellular concentrations of metabolites. The transport of cell nutrients represents the first step of many metabolic routes and may also regulate such processes. They are also responsible for reaching the effective intracellular concentration of therapeutic drugs and some mechanisms of resistance and tolerance also depend on them. However, the common techniques used to evaluate the metabolites transport in different cells types are not easy to carry out and require extensive training. In this chapter, we report detailed protocols and tips about the expression of transporters, different activity assays and transporter kinetics determination.

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Acknowledgments

This work was supported by Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, PIP 2010-0685, 2011-0263, and 2013-0664), Agencia Nacional de Promoción Científica y Tecnológica (FONCYT PICT 2012-0559, 2013-2218 and 2015-0539). CAP, MRM are members of the career of scientific investigator; CR and MS are research fellows from CONICET.

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Authors and Affiliations

  1. Laboratorio de Parasitología Molecular, Instituto de Investigaciones Médicas “A. Lanari”, IDIM-CONICET, Universidad de Buenos Aires, Combatientes de Malvinas, 3150, Buenos Aires, Argentina

    Claudio A. Pereira, Melisa Sayé, Chantal Reigada & Mariana R. Miranda

Authors
  1. Claudio A. Pereira
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  2. Melisa Sayé
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  3. Chantal Reigada
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  4. Mariana R. Miranda
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Corresponding author

Correspondence to Claudio A. Pereira .

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Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Pereira, C.A., Sayé, M., Reigada, C., Miranda, M.R. (2018). Techniques Used for Functional Characterization of Polyamine Transporters. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

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