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Determination of Arginine and Ornithine Decarboxylase Activities in Plants

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Book cover Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

In plants, putrescine is synthesized directly from the decarboxylation of ornithine and/or by the alternative arginine decarboxylase pathway. The prevalence of one or the other depends on the tissue and stress conditions. In both amino acid decarboxylation reactions, the corresponding enzymes use pyridoxal phosphate (PLP) as co-factor. PLP combines with the α-amino acid to form a Schiff base, which acts as substrate in the carboxyl group removal and CO2 formation. We describe the methodology employed for the determination of ODC and ADC activities in plant tissues by detecting the release of (C14) CO2 using (C14) labelled substrates (ornithine or arginine).

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Authors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Avda Joan XXIII 27-31, 08028, Barcelona, Spain

    Rubén Alcázar & Antonio F. Tiburcio

Authors
  1. Rubén Alcázar
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  2. Antonio F. Tiburcio
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Corresponding author

Correspondence to Rubén Alcázar .

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Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Alcázar, R., Tiburcio, A.F. (2018). Determination of Arginine and Ornithine Decarboxylase Activities in Plants. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

  • eBook Packages: Springer Protocols

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