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Synthetic Biology to Engineer Bacteriophage Genomes

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Bacteriophage Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1693))

Abstract

Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, targeted drug delivery, or even assembly of new materials.

In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes are addressed: a yeast-based platform and bacteriophage recombineering of electroporated DNA.

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Acknowledgements

This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project PTDC/BBB-BSS/6471/2014, the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), and under the scope of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462). This work was also supported by BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. Ana Rita Costa, Catarina Milho and Diana Priscila Pires acknowledge FCT for the grants SFRH/BPD/94648/2013, SFRH/BD/94434/2013 and SFRH/BPD/116187/2016, respectively.

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Authors and Affiliations

  1. LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, CEB – Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057, Braga, Portugal

    Ana Rita Costa, Catarina Milho, Joana Azeredo & Diana Priscila Pires

Authors
  1. Ana Rita Costa
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  2. Catarina Milho
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  3. Joana Azeredo
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  4. Diana Priscila Pires
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Corresponding author

Correspondence to Diana Priscila Pires .

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Editors and Affiliations

  1. LIBRO-Laboratory of Research in Biofilms Rosário Oliveira, CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Joana Azeredo

  2. CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Sanna Sillankorva

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Rita Costa, A., Milho, C., Azeredo, J., Pires, D.P. (2018). Synthetic Biology to Engineer Bacteriophage Genomes. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 1693. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7395-8_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7395-8_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7394-1

  • Online ISBN: 978-1-4939-7395-8

  • eBook Packages: Springer Protocols

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