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Bacteriophage Production in Bioreactors

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Book cover Bacteriophage Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1693))

Abstract

The optimal conditions for the production of virulent bacteriophages in bioreactors can vary greatly depending on the host–bacteriophage system used. We present a general method for the production of virulent bacteriophages in bioreactors that can be adapted to many host–bacteriophage systems and various operating conditions (reactor volume, medium composition, temperature, etc.). The procedures detail how to establish optimal initial infection conditions (infection load and initial multiplicity of infection (MOI)), prepare the host pre-culture and bioreactor, operate the bioreactor, and harvest the bacteriophage product. Batch operation is detailed but a short discussion addresses other modes of operation, namely two-stage continuous bioreactors and two-stage cycling bioreactors.

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Author information

Authors and Affiliations

  1. Chemical and Materials Engineering, University of Alberta, 12th floor Donadeo ICE Building, 9211 116 St NW, Edmonton, AB, Canada, T5M 0L5

    Maryam Agboluaje & Dominic Sauvageau

Authors
  1. Maryam Agboluaje
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  2. Dominic Sauvageau
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Corresponding author

Correspondence to Dominic Sauvageau .

Editor information

Editors and Affiliations

  1. LIBRO-Laboratory of Research in Biofilms Rosário Oliveira, CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Joana Azeredo

  2. CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Sanna Sillankorva

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Agboluaje, M., Sauvageau, D. (2018). Bacteriophage Production in Bioreactors. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 1693. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7395-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7395-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7394-1

  • Online ISBN: 978-1-4939-7395-8

  • eBook Packages: Springer Protocols

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