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Bacteriophage Therapy pp 123–137Cite as

In Vivo Bacteriophage Biodistribution

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1693))

Abstract

At the dawn of the renaissance of bacteriophage therapy, the full acceptation of bacteriophages as anti-bacterial agents requires the determination of their basic pharmacokinetic parameters. Such data, known for all conventional drugs used in human and veterinary medicine, allow optimizing dose regimens, efficacy, and help to limit toxicity. Here, we describe basic methods to experimentally obtain pharmacokinetic data and give also examples of data calculation to determine key parameters related to the biodistribution and elimination of bacteriophages in vivo.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Molecular Biology of Gene in Extremophiles, Institut Pasteur, 25 Rue du Docteur Roux, 75015, Paris, France

    Nicolas Dufour, Raphaëlle Delattre & Laurent Debarbieux

  2. Service de Réanimation Médico-Chirurgicale, Centre Hospitalier René Dubos, 6, avenue de l’île de France, 95300, Pontoise, France

    Nicolas Dufour

  3. INSERM, IAME, UMR 1137, 75018, Paris, France

    Nicolas Dufour & Raphaëlle Delattre

  4. Service d’Anesthésie-Réanimation, AP-HP, Hôpital Beaujon, 92110, Clichy, France

    Raphaëlle Delattre

Authors
  1. Nicolas Dufour
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  2. Raphaëlle Delattre
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  3. Laurent Debarbieux
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Corresponding author

Correspondence to Laurent Debarbieux .

Editor information

Editors and Affiliations

  1. LIBRO-Laboratory of Research in Biofilms Rosário Oliveira, CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Joana Azeredo

  2. CEB – Centre of Biological Engineering, University of Minho, Braga, Portugal

    Sanna Sillankorva

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Dufour, N., Delattre, R., Debarbieux, L. (2018). In Vivo Bacteriophage Biodistribution. In: Azeredo, J., Sillankorva, S. (eds) Bacteriophage Therapy. Methods in Molecular Biology, vol 1693. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7395-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7395-8_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7394-1

  • Online ISBN: 978-1-4939-7395-8

  • eBook Packages: Springer Protocols

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