Abstract
Imaging plant embryos at the cellular level over time is technically challenging, since the embryo, once its protective seed coat is removed, must be kept viable and unstressed on a microscope slide for the duration of the experiment. Here we describe a procedure and suitable apparatus for the visualization, over several days, of changes in endoplasmic reticulum (ER) morphology associated with the process of germination in Arabidopsis thaliana seeds. Moreover, we also present a user-friendly image analysis tool which enables subtle perturbations in the ER network to be measured.
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Dzimitrowicz, N., Breeze, E., Frigerio, L. (2018). Long-Term Imaging of Endoplasmic Reticulum Morphology in Embryos During Seed Germination. In: Hawes, C., Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum . Methods in Molecular Biology, vol 1691. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7389-7_6
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DOI: https://doi.org/10.1007/978-1-4939-7389-7_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7388-0
Online ISBN: 978-1-4939-7389-7
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