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Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques

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Book cover The Plant Endoplasmic Reticulum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1691))

Abstract

The plant endoplasmic reticulum (ER) forms several specialized structures. These include the sieve element reticulum (SER) and the desmotubule formed as the ER passes through plasmodesmata. Imaging both of these structures has been inhibited by the resolution limits of light microscopy and their relatively inaccessible locations, combined with the fragile nature of the ER. Here we describe methods to view desmotubules in live cells under 3D-structured illumination microscopy (3D-SIM) and methods to fix and prepare phloem tissue for both 3D-SIM and transmission electron microscopy (TEM) which preserve the fragile structure and allow the detailed imaging of the SER.

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Authors and Affiliations

  1. Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, Edinburgh, UK

    Karen Bell, Karl Oparka & Kirsten Knox

Authors
  1. Karen Bell
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  2. Karl Oparka
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  3. Kirsten Knox
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Corresponding author

Correspondence to Kirsten Knox .

Editor information

Editors and Affiliations

  1. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Chris Hawes

  2. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Verena Kriechbaumer

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Bell, K., Oparka, K., Knox, K. (2018). Preparation and Imaging of Specialized ER Using Super-Resolution and TEM Techniques. In: Hawes, C., Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum . Methods in Molecular Biology, vol 1691. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7389-7_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7389-7_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7388-0

  • Online ISBN: 978-1-4939-7389-7

  • eBook Packages: Springer Protocols

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