Abstract
Like in most eukaryotic cells, the plant endoplasmic reticulum (ER) network is physically linked to the plasma membrane (PM), forming ER-PM contact sites (EPCS). The protein complex required for maintaining the EPCS is composed of ER integral membrane proteins (e.g., VAP27, synaptotagmins), PM-associated proteins (e.g., NET3C), and the cytoskeleton. Here, we describe methods for identifying possible EPCS-associated proteins. These include GFP-tagged protein expression followed by image analysis, and immuno-gold labeling at the ultrastructural level. In combination, these methods can be used to identify the localization of putative EPCS proteins as well as used to postulate their subcellular function.
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Wang, P., Hawes, C., Richardson, C., Hussey, P.J. (2018). Characterization of Proteins Localized to Plant ER-PM Contact Sites. In: Hawes, C., Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum . Methods in Molecular Biology, vol 1691. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7389-7_3
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DOI: https://doi.org/10.1007/978-1-4939-7389-7_3
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7389-7
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