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Analysis of Protein Glycosylation in the ER

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The Plant Endoplasmic Reticulum

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1691))

Abstract

Protein N-glycosylation is an essential posttranslational modification which is initiated in the endoplasmic reticulum. In plants, the N-glycans play a pivotal role for protein folding and quality control. Through the interaction of glycan processing and binding reactions mediated by ER-resident glycosidases and specific carbohydrate binding proteins, the N-glycans contribute to the adoption of a native protein conformation. Properly folded glycoproteins are released from these processes and allowed to continue their transit to the Golgi where further processing and maturation of N-glycans leads to the formation of more complex structures with different functions. Incompletely folded glycoproteins are removed from the ER by a highly conserved degradation process to prevent the accumulation or secretion of misfolded proteins and maintain ER homeostasis. Here, we describe methods to analyze the N-glycosylation status and the glycan-dependent ER-associated degradation process in plants.

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Acknowledgements

We would like to thank Friedrich Altmann and Daniel Maresch (both Department of Chemistry) for LC-ESI-MS-analysis. This work was supported by grants from the Austrian Science Fund (FWF): P28218 and T655-B20.

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Authors and Affiliations

  1. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190, Vienna, Austria

    Jennifer Schoberer, Yun-Ji Shin, Ulrike Vavra, Christiane Veit & Richard Strasser

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  1. Jennifer Schoberer
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  2. Yun-Ji Shin
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  3. Ulrike Vavra
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  4. Christiane Veit
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  5. Richard Strasser
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Corresponding author

Correspondence to Richard Strasser .

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Editors and Affiliations

  1. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Chris Hawes

  2. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Verena Kriechbaumer

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Schoberer, J., Shin, YJ., Vavra, U., Veit, C., Strasser, R. (2018). Analysis of Protein Glycosylation in the ER. In: Hawes, C., Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum . Methods in Molecular Biology, vol 1691. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7389-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7389-7_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7388-0

  • Online ISBN: 978-1-4939-7389-7

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