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The Plant Endoplasmic Reticulum pp 125–137Cite as

ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1691))

Abstract

Plant ER membranes are the major site of biosynthesis of several lipid families (phospholipids, sphingolipids, neutral lipids such as sterols and triacylglycerols). The structural diversity of lipids presents considerable challenges to comprehensive lipid analysis. This chapter will briefly review the various biosynthetic pathways and will detail several aspects of the lipid analysis: lipid extraction, handling, separation, detection, identification, and data presentation. The different tools/approaches used for lipid analysis will also be discussed in relation to the studies to be carried out on lipid metabolism and function.

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Acknowledgments

Metabolome Facility-MetaboHUB (ANR-11-INBS-0010) of the Functional Genomic Center of Bordeaux.

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Authors and Affiliations

  1. CNRS-University of Bordeaux, UMR 5200 Membrane Biogenesis Laboratory, INRA Bordeaux Aquitaine, 33140, Villenave d’Ornon, France

    Laetitia Fouillen, Lilly Maneta-Peyret & Patrick Moreau

  2. MetaboHub-Metabolome Facility of Bordeaux, Functional Genomics Center, Bordeaux, France

    Laetitia Fouillen

  3. Bordeaux Imaging Center, UMS 3420 CNRS, US004 INSERM, University of Bordeaux, 33000, Bordeaux, France

    Patrick Moreau

Authors
  1. Laetitia Fouillen
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  2. Lilly Maneta-Peyret
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  3. Patrick Moreau
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Corresponding author

Correspondence to Patrick Moreau .

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Editors and Affiliations

  1. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Chris Hawes

  2. Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

    Verena Kriechbaumer

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Fouillen, L., Maneta-Peyret, L., Moreau, P. (2018). ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis. In: Hawes, C., Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum . Methods in Molecular Biology, vol 1691. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7389-7_10

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  • DOI: https://doi.org/10.1007/978-1-4939-7389-7_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7388-0

  • Online ISBN: 978-1-4939-7389-7

  • eBook Packages: Springer Protocols

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